•  Cattaneo Antonino

Group Members
Scientific Collaborations

Prof. Antonino Cattaneo
Neurotrophic Factors and Neurodegenerative Diseases

Group Leader


Education and Experiences

Degree in Physics (thesis in Biophysics), summa cum laude, University of Rome

Scuola Normale Superiore (Pisa) and CNR Institute of Neurophysiology (Pisa) Working on the coding of visual information in cells of the visual cortex

Junior staff scientist at the CNR Institute of Neurobiology in Rome Working on the mechanisms of action of Nerve Growth Factor in differentiating neurons

MRC Laboratory of Molecular Biology (Cambridge, UK) Working on antibody engineering for protein knock-out.

Group leader at the CNR Institute of Neurobiology in Rome Developed the intracellular antibody strategy, to ablate protein function with intracellularly targeted antibodies.

Full professor of Biophysics at the International School for Advanced Studies (SISSA) in Trieste, Italy.

Head of the Biophysiscs Sector of SISSA – involved in setting up from scratch the Neuroscience Program.

On sabbatical leave from SISSA, joined the MRC Laboratory of Molecular Biology (Cambridge UK)

Deputy Director of SISSA.

Founder of Lay Line Genomics, a biotech company spin off of SISSA.

Associate Scientific Director at the European Brain Research Institute (EBRI) Rome, Italy AWARDS Member of the European Molecular Biology Organization (EMBO) Member of the Council of Scientists of Human Science Frontier Organization (HFSPO) and served for four years as the Chairman of the Grants Review Committee. Associate Editor of the Journal of Alzheimer’s Disease and ad hoc reviewer for leading international scientific journals. Member of the Scientific Program Committee of the International Conference on Alzheimer’s Disease and Related Disorders.


Paoletti F, Covaceuszach S, Konarev PV, Gonfloni S, Malerba F, Schwarz E, Svergun DI, Cattaneo A, Lamba D (2009) Intrinsic structural disorder of mouse proNGF. Proteins 75, 990-1009.

Origlia N, Capsoni S, Cattaneo A, Fang F, Arancio O, Yan SD, Domenici L (2009) Abeta-dependent Inhibition of LTP in different intracortical circuits of the visual cortex: the role of RAGE. J Alzheimers Dis 17, 59-68.

Meli G, Visintin M, Cannistraci I, Cattaneo A (2009) Direct in vivo intracellular selection of conformation-sensitive antibody domains targeting Alzheimer´s amyloid-beta oligomers. J Mol Biol 387, 584-606.

D´Onofrio M, Arisi I, Brandi R, Di Mambro A, Felsani A, S C, Cattaneo A (2009) Early inflammation and immune response mRNAs in the brain of AD11 anti-NGF mice. Neurobiol Aging (in press).

Covaceuszach S, Capsoni S, Ugolini G, Spirito F, Vignone D, Cattaneo A (2009) Development of a non invasive NGF-based therapy for Alzheimer´s disease. Curr Alzheimer Res 6, 158-170.

Capsoni S, Covaceuszach S, Ugolini G, Spirito F, Vignone D, Stefanini B, Amato G, Cattaneo A (2009) Delivery of NGF to the brain: intranasal versus ocular administration in anti-NGF transgenic mice. J Alzheimers Dis 16, 371-388.

Zacchi P, Dreosti E, Visintin M, Moretto-Zita M, Marchionni I, Cannistraci I, Kasap Z, Betz H, Cattaneo A, Cherubini E (2008) Gephyrin selective intrabodies as a new strategy for studying inhibitory receptor clustering. J Mol Neurosci 34, 141-148.

Visintin M, Melchionna T, Cannistraci I, Cattaneo A (2008) In vivo selection of intrabodies specifically targeting protein-protein interactions: a general platform for an "undruggable" class of disease targets. J Biotechnol 135, 1-15.

Terzibasi E, Valenzano DR, Benedetti M, Roncaglia P, Cattaneo A, Domenici L, Cellerino A (2008) Large differences in aging phenotype between strains of the short-lived annual fish Nothobranchius furzeri. PLoS One 3, e3866.

Origlia N, Righi M, Capsoni S, Cattaneo A, Fang F, Stern DM, Chen JX, Schmidt AM, Arancio O, Yan SD, Domenici L (2008) Receptor for advanced glycation end product-dependent activation of p38 mitogen-activated protein kinase contributes to amyloid-beta-mediated cortical synaptic dysfunction. J Neurosci 28, 3521-3530.

Matrone C, Di Luzio A, Meli G, D´Aguanno S, Severini C, Ciotti MT, Cattaneo A, Calissano P (2008) Activation of the amyloidogenic route by NGF deprivation induces apoptotic death in PC12 cells. J Alzheimers Dis 13, 81-96.

Covaceuszach S, Cassetta A, Konarev PV, Gonfloni S, Rudolph R, Svergun DI, Lamba D, Cattaneo A (2008) Dissecting NGF interactions with TrkA and p75 receptors by structural and functional studies of an anti-NGF neutralizing antibody. J Mol Biol 381, 881-896.

Corsetti V, Amadoro G, Gentile A, Capsoni S, Ciotti MT, Cencioni MT, Atlante A, Canu N, Rohn TT, Cattaneo A, Calissano P (2008) Identification of a caspase-derived N-terminal tau fragment in cellular and animal Alzheimer´s disease models. Mol Cell Neurosci 38, 381-392.

Cattaneo A, Capsoni S, Paoletti F (2008) Towards non invasive Nerve Growth Factor therapies for Alzheimer´s disease. Journal of Alzheimer´s disease 15, 255-283.

Ugolini G, Marinelli S, Covaceuszach S, Cattaneo A, Pavone F (2007) The function neutralizing anti-TrkA antibody MNAC13 reduces inflammatory and neuropathic pain. Proc Natl Acad Sci U S A 104, 2985-2990.

Melchionna T, Cattaneo A (2007) A protein silencing switch by ligand-induced proteasome-targeting intrabodies. J Mol Biol 374, 641-654.

Cavalli A, Bolognesi ML, Capsoni S, Andrisano V, Bartolini M, Margotti E, Cattaneo A, Recanatini M, Melchiorre C (2007) A small molecule targeting the multifactorial nature of Alzheimer´s disease. Angew Chem Int Ed Engl 46, 3689-3692.

Bolognesi ML, Banzi R, Bartolini M, Cavalli A, Tarozzi A, Andrisano V, Minarini A, Rosini M, Tumiatti V, Bergamini C, Fato R, Lenaz G, Hrelia P, Cattaneo A, Recanatini M, Melchiorre C (2007) Novel Class of Quinone-Bearing Polyamines as Multi-Target-Di rected Ligands To Combat Alzheimer´s Disease. J Med Chem.

Berardi N, Braschi C, Capsoni S, Cattaneo A, Maffei L (2007) Environmental enrichment delays the onset of memory deficits and reduces neuropathological hallmarks in a mouse model of Alzheimer-like neurodegeneration. J Alzheimers Dis 11, 359-370.

Valenzano DR, Terzibasi E, Genade T, Cattaneo A, Domenici L, Cellerino A (2006) Resveratrol prolongs lifespan and retards the onset of age-related markers in a short-lived vertebrate. Curr Biol 16, 296-300.

Valenzano DR, Terzibasi E, Cattaneo A, Domenici L, Cellerino A (2006) Temperature affects longevity and age-related locomotor and cognitive decay in the short-lived fish Nothobranchius furzeri. Aging Cell 5, 275-278.

Sola E, Capsoni S, Rosato-Siri M, Cattaneo A, Cherubini E (2006) Failure of nicotine-dependent enhancement of synaptic efficacy at Schaffer-collateral CA1 synapses of AD11 anti-nerve growth factor transgenic mice. Eur J Neurosci 24, 1252-1264.

Rosato-Siri M, Cattaneo A, Cherubini E (2006) Nicotine-induced enhancement of synaptic plasticity at CA3-CA1 synapses requires GABAergic interneurons in adult anti-NGF mice. J Physiol 576, 361-377.

Rende M, Pistilli A, Stabile AM, Terenzi A, Cattaneo A, Ugolini G, Sanna P (2006) Role of nerve growth factor and its receptors in non-nervous cancer growth: efficacy of a tyrosine kinase inhibitor (AG879) and neutralizing antibodies antityrosine kinase receptor A and antinerve growth factor: an in-vitro and in-vivo study. Anticancer Drugs 17, 929-941.

Paoletti F, Konarev PV, Covaceuszach S, Schwarz E, Cattaneo A, Lamba D, Svergun DI (2006) Structural and functional properties of mouse proNGF. Biochem Soc Trans 34, 605-606.

Origlia N, Capsoni S, Domenici L, Cattaneo A (2006) Time window in cholinomimetic ability to rescue long-term potentiation in neurodegenerating anti-nerve growth factor mice. J Alzheimers Dis 9, 59-68.

Capsoni S, Cattaneo A (2006) On the molecular basis linking Nerve Growth Factor (NGF) to Alzheimer´s disease. Cell Mol Neurobiol 26, 619-633.

Berrera M, Cattaneo A, Carloni P (2006) Molecular simulation of the binding of nerve growth factor peptide mimics to the receptor tyrosine kinase A. Biophys J 91, 2063-2071.

Arisi I, Cattaneo A, Rosato V (2006) Parameter estimate of signal transduction pathways. BMC Neurosci 7 Suppl 1, S6.

Vascotto F, Visintin M, Cattaneo A, Burrone OR (2005) Design and selection of an intrabody library produced de-novo for the non-structural protein NSP5 of rotavirus. J Immunol Methods 301, 31-40.

Pattabiraman PP, Tropea D, Chiaruttini C, Tongiorgi E, Cattaneo A, Domenici L (2005) Neuronal activity regulates the developmental expression and subcellular localization of cortical BDNF mRNA isoforms in vivo. Mol Cell Neurosci 28, 556-570.

Mladinic M, Wintzer M, Del Bel E, Casseler C, Lazarevic D, Crovella S, Gustincich S, Cattaneo A, Nicholls J (2005) Differential expression of genes at stages when regeneration can and cannot occur after injury to immature mammalian spinal cord. Cell Mol Neurobiol 25, 407-426.

Genade T, Benedetti M, Terzibasi E, Roncaglia P, Valenzano DR, Cattaneo A, Cellerino A (2005) Annual fishes of the genus Nothobranchius as a model system for aging research. Aging Cell 4, 223-233.

Fasulo L, Ugolini G, Cattaneo A (2005) Apoptotic effect of caspase-3 cleaved tau in hippocampal neurons and its potentiation by tau FTDP-mutation N279K. J Alzheimers Dis 7, 3-13.

De Rosa R, Garcia AA, Braschi C, Capsoni S, Maffei L, Berardi N, Cattaneo A (2005) Intranasal administration of nerve growth factor (NGF) rescues recognition memory deficits in AD11 anti-NGF transgenic mice. Proc Natl Acad Sci U S A 102, 3811-3816.

Covaceuszach S, Cattaneo A, Lamba D (2005) Neutralization of NGF-TrkA receptor interaction by the novel antagonistic anti-TrkA monoclonal antibody MNAC13: a structural insight. Proteins 58, 717-727.

Wintzer M, Mladinic M, Lazarevic D, Casseler C, Cattaneo A, Nicholls J (2004) Strategies for identifying genes that play a role in spinal cord regeneration. J Anat 204, 3-11.

Visintin M, Quondam M, Cattaneo A (2004) The intracellular antibody capture technology: towards the high-throughput selection of functional intracellular antibodies for target validation. Methods 34, 200-214.

Visintin M, Meli GA, Cannistraci I, Cattaneo A (2004) Intracellular antibodies for proteomics. J Immunol Methods 290, 135-153.

Vascotto F, Campagna M, Visintin M, Cattaneo A, Burrone OR (2004) Effects of intrabodies specific for rotavirus NSP5 during the virus replicative cycle. J Gen Virol 85, 3285-3290.

Tongiorgi E, Armellin M, Giulianini PG, Bregola G, Zucchini S, Paradiso B, Steward O, Cattaneo A, Simonato M (2004) Brain-derived neurotrophic factor mRNA and protein are targeted to discrete dendritic laminas by events that trigger epileptogenesis. J Neurosci 24, 6842-6852.

Covaceuszach S, Cassetta A, Cattaneo A, Lamba D (2004) Purification, crystallization, X-ray diffraction analysis and phasing of a Fab fragment of monoclonal neuroantibody alphaD11 against nerve growth factor. Acta Crystallogr D Biol Crystallogr 60, 1323-1327.

Capsoni S, Giannotta S, Stebel M, Garcia AA, De Rosa R, Villetti G, Imbimbo BP, Pietra C, Cattaneo A (2004) Ganstigmine and donepezil improve neurodegeneration in AD11 antinerve growth factor transgenic mice. Am J Alzheimers Dis Other Demen 19, 153-160.

Brancucci A, Kuczewski N, Covaceuszach S, Cattaneo A, Domenici L (2004) Nerve growth factor favours long-term depression over long-term potentiation in layer II-III neurones of rat visual cortex. J Physiol 559, 497-506.

Tongiorgi E, Ferrero F, Cattaneo A, Domenici L (2003) Dark-rearing decreases NR2A N-methyl-D-aspartate receptor subunit in all visual cortical layers. Neuroscience 119, 1013-1022.

Sulpizi M, Laio A, VandeVondele J, Cattaneo A, Rothlisberger U, Carloni P (2003) Reaction mechanism of caspases: insights from QM/MM Car-Parrinello simulations. Proteins 52, 212-224.

Settanni G, Cattaneo A, Carloni P (2003) Molecular dynamics simulations of the NGF-TrkA domain 5 complex and comparison with biological data. Biophys J 84, 2282-2292.

Corvetti L, Capsoni S, Cattaneo A, Domenici L (2003) Postnatal development of GFAP in mouse visual cortex is not affected by light deprivation. Glia 41, 404-414.

Visintin M, Settanni G, Maritan A, Graziosi S, Marks JD, Cattaneo A (2002) The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies. J Mol Biol 317, 73-83.

Tropea D, Capsoni S, Covaceuszach S, Domenici L, Cattaneo A (2002) Rat visual cortical neurones express TrkA NGF receptor. Neuroreport 13, 1369-1373.

Pesavento E, Capsoni S, Domenici L, Cattaneo A (2002) Acute cholinergic rescue of synaptic plasticity in the neurodegenerating cortex of anti-nerve-growth-factor mice. Eur J Neurosci 15, 1030-1036.

Margotti E, Covaceuszach S, Tongiorgi E, Cattaneo A, Domenici L (2002) TRKB signalling controls the expression of N-methyl-d-aspartate receptors in the visual cortex. Eur J Neurosci 16, 1067-1074.

Dauvillier S, Merida P, Visintin M, Cattaneo A, Bonnerot C, Dariavach P (2002) Intracellular single-chain variable fragments directed to the Src homology 2 domains of Syk partially inhibit Fc epsilon RI signaling in the RBL-2H3 cell line. J Immunol 169, 2274-2283.

Capsoni S, Giannotta S, Cattaneo A (2002) Early events in Alzheimer-like neurodegeneration in anti-nerve growth factor transgenic mice. Brain Aging, 24-43.

Capsoni S, Giannotta S, Cattaneo A (2002) Beta-amyloid plaques in a model for sporadic Alzheimer´s disease based on transgenic anti-nerve growth factor antibodies. Mol Cell Neurosci 21, 15-28.

Capsoni S, Giannotta S, Cattaneo A (2002) Nerve growth factor and galantamine ameliorate early signs of neurodegeneration in anti-nerve growth factor mice. Proc Natl Acad Sci U S A 99, 12432-12437.

Tropea D, Capsoni S, Tongiorgi E, Giannotta S, Cattaneo A, Domenici L (2001) Mismatch between BDNF mRNA and protein expression in the developing visual cortex: the role of visual experience. Eur J Neurosci 13, 709-721.

Settanni G, Cattaneo A, Maritan A (2001) Role of native-state topology in the stabilization of intracellular antibodies. Biophys J 81, 2935-2945.

Covaceuszach S, Cattaneo A, Lamba D (2001) Purification, crystallization and preliminary X-ray analysis of the Fab fragment from MNAC13, a novel antagonistic anti-tyrosine kinase A receptor monoclonal antibody. Acta Crystallogr D Biol Crystallogr 57, 1307-1309.

Tongiorgi E, Armellin M, Cattaneo A (2000) Differential somato-dendritic localization of TrkA, TrkB, TrkC and p75 mRNAs in vivo. Neuroreport 11, 3265-3268.

Sermasi E, Margotti E, Cattaneo A, Domenici L (2000) Trk B signalling controls LTP but not LTD expression in the developing rat visual cortex. Eur J Neurosci 12, 1411-1419.

Ruberti F, Capsoni S, Comparini A, Di Daniel E, Franzot J, Gonfloni S, Rossi G, Berardi N, Cattaneo A (2000) Phenotypic knockout of nerve growth factor in adult transgenic mice reveals severe deficits in basal forebrain cholinergic neurons, cell death in the spleen, and skeletal muscle dystrophy. J Neurosci 20, 2589-2601.

Righi M, Tongiorgi E, Cattaneo A (2000) Brain-derived neurotrophic factor (BDNF) induces dendritic targeting of BDNF and tyrosine kinase B mRNAs in hippocampal neurons through a phosphatidylinositol-3 kinase-dependent pathway. J Neurosci 20, 3165-3174.

Pesavento E, Margotti E, Righi M, Cattaneo A, Domenici L (2000) Blocking the NGF-TrkA interaction rescues the developmental loss of LTP in the rat visual cortex: role of the cholinergic system. Neuron 25, 165-175.

Lener M, Horn IR, Cardinale A, Messina S, Nielsen UB, Rybak SM, Hoogenboom HR, Cattaneo A, Biocca S (2000) Diverting a protein from its cellular location by intracellular antibodies. The case of p21Ras. Eur J Biochem 267, 1196-1205.

Fasulo L, Ugolini G, Visintin M, Bradbury A, Brancolini C, Verzillo V, Novak M, Cattaneo A (2000) The neuronal microtubule-associated protein tau is a substrate for caspase-3 and an effector of apoptosis. J Neurochem 75, 624-633.

Esposito G, Viglino P, Novak M, Cattaneo A (2000) The solution structure of the C-terminal segment of tau protein. J Pept Sci 6, 550-559.

Capsoni S, Ugolini G, Comparini A, Ruberti F, Berardi N, Cattaneo A (2000) Alzheimer-like neurodegeneration in aged antinerve growth factor transgenic mice. Proc Natl Acad Sci U S A 97, 6826-6831.

Capsoni S, Ruberti F, Di Daniel E, Cattaneo A (2000) Muscular dystrophy in adult and aged anti-NGF transgenic mice resembles an inclusion body myopathy. J Neurosci Res 59, 553-560.

Visintin M, Tse E, Axelson H, Rabbitts TH, Cattaneo A (1999) Selection of antibodies for intracellular function using a two-hybrid in vivo system. Proc Natl Acad Sci U S A 96, 11723-11728.

Tongiorgi E, Cattaneo A, Domenici L (1999) Co-expression of TrkB and the N-methyl-D-aspartate receptor subunits NR1-C1, NR2A and NR2B in the rat visual cortex. Neuroscience 90, 1361-1369.

Rosati AM, Guarnieri E, Avignone E, Cherubini E, Cattaneo A, Traversa U (1999) Increased density of M1 receptors in the hippocampus of juvenile rats chronically deprived of NGF. Brain Res 815, 185-191.

Persic L, Horn IR, Rybak S, Cattaneo A, Hoogenboom HR, Bradbury A (1999) Single-chain variable fragments selected on the 57-76 p21Ras neutralising epitope from phage antibody libraries recognise the parental protein. FEBS Lett 443, 112-116.

Cattaneo A, Capsoni S, Margotti E, Righi M, Kontsekova E, Pavlik P, Filipcik P, Novak M (1999) Functional blockade of tyrosine kinase A in the rat basal forebrain by a novel antagonistic anti-receptor monoclonal antibody. J Neurosci 19, 9687-9697.

Cattaneo A, Biocca S (1999) The selection of intracellular antibodies. Trends Biotechnol 17, 115-121.

Capsoni S, Tongiorgi E, Cattaneo A, Domenici L (1999) Dark rearing blocks the developmental down-regulation of brain-derived neurotrophic factor messenger RNA expression in layers IV and V of the rat visual cortex. Neuroscience 88, 393-403.

Capsoni S, Tongiorgi E, Cattaneo A, Domenici L (1999) Differential regulation of brain-derived neurotrophic factor messenger RNA cellular expression in the adult rat visual cortex. Neuroscience 93, 1033-1040.

Tongiorgi E, Righi M, Cattaneo A (1998) A non-radioactive in situ hybridization method that does not require RNAse-free conditions. J Neurosci Methods 85, 129-139.

Molnar M, Tongiorgi E, Avignone E, Gonfloni S, Ruberti F, Domenici L, Cattaneo A (1998) The effects of anti-nerve growth factor monoclonal antibodies on developing basal forebrain neurons are transient and reversible. Eur J Neurosci 10, 3127-3140.

Cattaneo A (1998) Selection of intracellular antibodies. Bratisl Lek Listy 99, 413-418.

Cardinale A, Lener M, Messina S, Cattaneo A, Biocca S (1998) The mode of action of Y13-259 scFv fragment intracellularly expressed in mammalian cells. FEBS Lett 439, 197-202.

Canu N, Dus L, Barbato C, Ciotti MT, Brancolini C, Rinaldi AM, Novak M, Cattaneo A, Bradbury A, Calissano P (1998) Tau cleavage and dephosphorylation in cerebellar granule neurons undergoing apoptosis. J Neurosci 18, 7061-7074.

Biocca S, Tafani M, Cattaneo A (1998) Assembled IgG molecules are exported from the endoplasmic reticulum in myeloma cells despite the retention signal SEKDEL. Biochem Biophys Res Commun 246, 518-523.

Avignone E, Molnar M, Berretta N, Casamenti F, Prosperi C, Ruberti F, Cattaneo A, Cherubini E (1998) Cholinergic function in the hippocampus of juvenile rats chronically deprived of NGF. Brain Res Dev Brain Res 109, 137-147.

Ugolini G, Cattaneo A, Novak M (1997) Co-localization of truncated tau and DNA fragmentation in Alzheimer´s disease neurones. Neuroreport 8, 3709-3712.

Tongiorgi E, Righi M, Cattaneo A (1997) Activity-dependent dendritic targeting of BDNF and TrkB mRNAs in hippocampal neurons. J Neurosci 17, 9492-9505.

Ruberti F, Berretta N, Cattaneo A, Cherubini E (1997) NGF antibodies impair long-term depression at the mossy fibre-CA3 synapse in the developing hippocampus. Brain Res Dev Brain Res 101, 295-297.

Persic L, Roberts A, Wilton J, Cattaneo A, Bradbury A, Hoogenboom HR (1997) An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries. Gene 187, 9-18.

Persic L, Righi M, Roberts A, Hoogenboom HR, Cattaneo A, Bradbury A (1997) Targeting vectors for intracellular immunisation. Gene 187, 1-8.

Molnar M, Ruberti F, Cozzari C, Domenici L, Cattaneo A (1997) A critical period in the sensitivity of basal forebrain cholinergic neurones to NGF deprivation. Neuroreport 8, 575-579.

Gargano N, Cattaneo A (1997) Rescue of a neutralizing anti-viral antibody fragment from an intracellular polyclonal repertoire expressed in mammalian cells. FEBS Lett 414, 537-540.

Gargano N, Cattaneo A (1997) Inhibition of murine leukaemia virus retrotranscription by the intracellular expression of a phage-derived anti-reverse transcriptase antibody fragment. J Gen Virol 78 ( Pt 10), 2591-2599.

Bertollini L, Ciotti MT, Cherubini E, Cattaneo A (1997) Neurotrophin-3 promotes the survival of oligodendrocyte precursors in embryonic hippocampal cultures under chemically defined conditions. Brain Res 746, 19-24.

Tongiorgi E, Righi M, Cattaneo A (1996) Subcellular localisation of neurotrophins and neurotrophin receptors: implications for synaptic plasticity. Rev Bras Biol 56 Su 1 Pt 1, 175-182.

Gargano N, Biocca S, Bradbury A, Cattaneo A (1996) Human recombinant antibody fragments neutralizing human immunodeficiency virus type 1 reverse transcriptase provide an experimental basis for the structural classification of the DNA polymerase family. J Virol 70, 7706-7712.

Piccioli P, Di Luzio A, Amann R, Schuligoi R, Surani MA, Donnerer J, Cattaneo A (1995) Neuroantibodies: ectopic expression of a recombinant anti-substance P antibody in the central nervous system of transgenic mice. Neuron 15, 373-384.

Kontsekova E, Cattaneo A, Novak M (1995) Quick purification of recombinant human truncated tau proteins for immunoanalysis. J Immunol Methods 185, 245-248.

Bradbury A, Cattaneo A (1995) The use of phage display in neurobiology. Trends Neurosci 18, 243-249.

Biocca S, Ruberti F, Tafani M, Pierandrei-Amaldi P, Cattaneo A (1995) Redox state of single chain Fv fragments targeted to the endoplasmic reticulum, cytosol and mitochondria. Biotechnology (N Y) 13, 1110-1115.

Biocca S, Cattaneo A (1995) Intracellular immunization: antibody targeting to subcellular compartments. Trends Cell Biol 5, 248-252.

Bertollini L, Cattaneo A, Cherubini E (1995) Neurofilament-negative neurones exhibit HVA Ca2+ currents with faster inactivation kinetics. Neuroreport 6, 857-860.

Amati V, Werge TM, Cattaneo A, Tramontano A (1995) Identifying a putative common binding site shared by substance P receptor and an anti-substance P monoclonal antibody. Protein Eng 8, 403-408.

Russo MA, Odorisio T, Fradeani A, Rienzi L, De Felici M, Cattaneo A, Siracusa G (1994) Low-affinity nerve growth factor receptor is expressed during testicular morphogenesis and in germ cells at specific stages of spermatogenesis. Mol Reprod Dev 37, 157-166.

Ruberti F, Cattaneo A, Bradbury A (1994) The use of the RACE method to clone hybridoma cDNA when V region primers fail. J Immunol Methods 173, 33-39.

Domenici L, Fontanesi G, Cattaneo A, Bagnoli P, Maffei L (1994) Nerve growth factor (NGF) uptake and transport following injection in the developing rat visual cortex. Vis Neurosci 11, 1093-1102.

Domenici L, Cellerino A, Berardi N, Cattaneo A, Maffei L (1994) Antibodies to nerve growth factor (NGF) prolong the sensitive period for monocular deprivation in the rat. Neuroreport 5, 2041-2044.

Biocca S, Pierandrei-Amaldi P, Campioni N, Cattaneo A (1994) Intracellular immunization with cytosolic recombinant antibodies. Biotechnology (N Y) 12, 396-399.

Berardi N, Cellerino A, Domenici L, Fagiolini M, Pizzorusso T, Cattaneo A, Maffei L (1994) Monoclonal antibodies to nerve growth factor affect the postnatal development of the visual system. Proc Natl Acad Sci U S A 91, 684-688.

Tavladoraki P, Benvenuto E, Trinca S, De Martinis D, Cattaneo A, Galeffi P (1993) Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature 366, 469-472.

Ruberti F, Bradbury A, Cattaneo A (1993) Cloning and expression of an anti-nerve growth factor (NGF) antibody for studies using the neuroantibody approach. Cell Mol Neurobiol 13, 559-568.

Greco A, Orlandi R, Mariani C, Miranda C, Borrello MG, Cattaneo A, Pagliardini S, Pierotti MA (1993) Expression of TRK-T1 oncogene induces differentiation of PC12 cells. Cell Growth Differ 4, 539-546.

Bradbury A, Persic L, Werge T, Cattaneo A (1993) Use of living columns to select specific phage antibodies. Biotechnology (N Y) 11, 1565-1569.

Biocca S, Pierandrei-Amaldi P, Cattaneo A (1993) Intracellular expression of anti-p21ras single chain Fv fragments inhibits meiotic maturation of xenopus oocytes. Biochem Biophys Res Commun 197, 422-427.

Werge TM, Bradbury A, Di Luzio A, Cattaneo A (1992) A recombinant cell line expressing a form of the Y13-259 anti-p21ras antibody which binds protein A and may be produced as ascites. Oncogene 7, 1033-1035.

Piccioli P, Ruberti F, Biocca S, Di Luzio A, Werge TM, Bradbury A, Cattaneo A (1991) Neuroantibodies: molecular cloning of a monoclonal antibody against substance P for expression in the central nervous system. Proc Natl Acad Sci U S A 88, 5611-5615.

Biocca S, Di Luzio A, Werge T, Cattaneo A (1991) Intracellular immunization: expression of antibody domains in the cytoplasm and in the nucleus of mammalian cells. Cytotechnology 5 Suppl 1, 49-50.

Benvenuto E, Ordas RJ, Tavazza R, Ancora G, Biocca S, Cattaneo A, Galeffi P (1991) ´Phytoantibodies´: a general vector for the expression of immunoglobulin domains in transgenic plants. Plant Mol Biol 17, 865-874.

Werge TM, Biocca S, Cattaneo A (1990) Intracellular immunization. Cloning and intracellular expression of a monoclonal antibody to the p21ras protein. FEBS Lett 274, 193-198.

Biocca S, Neuberger MS, Cattaneo A (1990) Expression and targeting of intracellular antibodies in mammalian cells. Embo J 9, 101-108.

Levi A, Biocca S, Cattaneo A, Calissano P (1988) The mode of action of nerve growth factor in PC12 cells. Mol Neurobiol 2, 201-226.

Cattaneo A, Rapposelli B, Calissano P (1988) Three distinct types of monoclonal antibodies after long-term immunization of rats with mouse nerve growth factor. J Neurochem 50, 1003-1010.

Cattaneo A (1988) [Neuroantibodies: expression of specific monoclonal antibodies in cells of the nervous system]. Ann Ist Super Sanita 24, 531-533.

Cattaneo A, Neuberger MS (1987) Polymeric immunoglobulin M is secreted by transfectants of non-lymphoid cells in the absence of immunoglobulin J chain. EMBO J 6, 2753-2758.

Cattaneo A, Biocca S, Corvaja N, Calissano P (1985) Nuclear localization of a lactic dehydrogenase with single-stranded DNA-binding properties. Exp Cell Res 161, 130-140.

Calissano P, Volonte C, Biocca S, Cattaneo A (1985) Synthesis and content of a DNA-binding protein with lactic dehydrogenase activity are reduced by nerve growth factor in the neoplastic cell line PC12. Exp Cell Res 161, 117-129.

Calissano P, Cattaneo A, Biocca S, Aloe L, Mercanti D, Levi-Montalcini R (1984) The nerve growth factor. Established findings and controversial aspects. Exp Cell Res 154, 1-9.

Biocca S, Cattaneo A, Calissano P (1984) Nerve growth factor inhibits the synthesis of a single-stranded DNA binding protein in pheochromocytoma cells (clone PC12). Proc Natl Acad Sci U S A 81, 2080-2084.

Spinelli D, Cattaneo A (1983) Contrast summation in dichoptic vision. Psychol Res 45, 1-10.

Cattaneo A, Biocca S, Nasi S, Calissano P (1983) Hidden receptors for nerve growth factor in PC12 cells. Eur J Biochem 135, 285-290.

Biocca S, Cattaneo A, Calissano P (1983) A macromolecular structure favouring microtubule assembly in NGF-differentiated pheochromocytoma cells (PC12). Embo J 2, 643-648.

Corvaja N, Di Luzio A, Biocca S, Cattaneo A, Calissano P (1982) Morphological and ultrastructural changes in PC12 pheochromocytoma cells induced by a combined treatment with NGF and taxol. Exp Cell Res 142, 385-395.

Cattaneo A, Spinelli D (1982) Contrast influence on perceived orientation. Vision Res 22, 783-785.

Berardi N, Bisti S, Cattaneo A, Fiorentini A, Maffei L (1982) Correlation between the preferred orientation and spatial frequency of neurones in visual areas 17 and 18 of the cat. J Physiol 323, 603-618.

Cattaneo A, Maffei L, Morrone C (1981) Two firing patterns in the discharge of complex cells encoding different attributes of the visual stimulus. Exp Brain Res 43, 115-118.

Cattaneo A, Maffei L, Morrone C (1981) Patterns in the discharge of simple and complex visual cortical cells. Proc R Soc Lond B Biol Sci 212, 279-297.

Berardi N, Bisti S, Cattaneo A, Fiorentini A, Maffei L (1979) Spatial frequency rows in area 18 of the cat [proceedings]. J Physiol 292, 29P-30P.


The Domenico Marotta Prize, Italian Academy of Sciences XL

The W. Jansenius Medal, Slovak Academy of Sciences

Scientific Collaborations

Group Members

Mara D´Onofrio, Luisa Fasulo, Raffaella Scardigli, Simona Capsoni, Giovanni Meli, Francesca Paoletti, Francesca Malerba, Marcello Ceci, Annalisa Manca, Gianluca Amato, Agnese Lecci

Laboratorio di Fattori Neurotrofici e Malattie Neurodegenerative 


Mara D´Onofrio, M.D., Ph.D.
Also at Genomics Facility

She worked at NIH, Bethesda (USA), working on nuclear transport, where she isolated and characterized the gene coding for the Nuclear Pore Glycoprotein, p62. Her recent focus has been on metabotropic glutamate receptors as potential targets for neuroprotective drugs in the treatment of neurodegenerative disorders.

D´Onofrio M, Starr CM, Park MK, Holt GD, Haltiwanger GS, Hart GW, Hanover JA (1988) Partial cDNA sequence encoding a nuclear pore protein modified by O-linked N-acetylglucosamine. Proc Natl Acad Sci, 85: 9595-9599.

Starr CM, D´Onofrio M, Park MK, Hanover JA (1990) Primary sequence and heterologous expression of Nuclear Pore glycoprotein p62. J Cell Biology, 110: 1861-1871.

D´Onofrio M, Lee M.D., Starr CM, Miller M, Hanover JA (1991) The gene encoding rat nuclear pore glycoprotein p62 is intronless. J Biol Chemistry, 266: 11980-11985.

Besong G*, Battaglia G*, D´Onofrio M*, Di Marco R, Ngomba RT, Storto M, Castiglione M, Mangano K, Buscati CL, Nicoletti FR, Bacon K, Tusche M, Valenti O, Conn PJ, Bruno V and Nicoletti F (2002) Activation of group-III metabotropic glutamate receptors inhibits the production of RANTES in glial cell culture. J Neurosci, 22:5403-5411. *Equal contributors

D´Onofrio M, Arcella A, Bruno V, Battaglia G, Ngomba RT, Lombari V, Ragona G, Calogero A and Nicoletti F (2003) Pharmacological blockade of mGlu2/3 metabotropic glutamate receptors reduces cell proliferation in cultured human glioma cells. J Neurochem, 84:1288-1295.

Luisa Fasulo, M.D., Ph.D.

The main research interests are the development of cellular models of neurodegeneration, as well as strategies for novel ex vivo cell therapy or gene therapy approaches for Alzheimer´s Disease.

Fasulo L, Ugolini G and Cattaneo A. (2005) Apoptotic effect of caspase-3 cleaved tau in hippocampal neurons and its potentiation by tau FTDP-mutation N279K. J Alzheimers Dis, 7(1):3-13.

Fasulo L, Ugolini G, Visintin M, Bradbury A, Brancolini C, Verzillo V, Novak M, and A Cattaneo (2000) The neuronal microtubule-associated protein tau is a substrate for caspase-3 and an effector of apoptosis. J Neurochem, 75(2):624-33.

Raffaella Scardigli - Research Scientist

Simona Capsoni - Research Scientist

Giovanni Meli - Postdoctoral Fellow

His project is focused on the selection and characterization of a novel generation of recombinant antibodies against beta-Amyloid oligomers.

Visintin M, Meli GA, Cannistraci I, Cattaneo A. (2004) Intracellular antibodies for proteomics. J Immunol Methods 290(1-2):135-53

Meli G, Visintin M, Cannistraci I, Westlind-Danielsson A, Cattaneo A. (2005) Novel generation of recombinant antibodies against oligomeric form of beta-Amyloid 1-42 peptide Society for Neuroscience Abstract No. 325.8

Francesca Paoletti - Postdoctoral Fellow

Her current project is the elucidation of the structure and function of proNGF, in order to better explain the molecular basis of the neurodegenerative processes based on the dysfunction of NGF.

Paoletti F., Konarev P.V., Covaceuszach S., Schwarz E., Cattaneo A., Lamba D., Svergun D.I. (2006) Structural and functional properties of mouse proNGF. Biochem Soc Trans. 2006 Aug;34(Pt4):605-6.

Paoletti F., Covaceuszach S., Schwarz E., Stubbs M., Rudolph R., Cattaneo A. and Lamba D. (2005) Towards a comprehension of the structure of mouse proNGF. Acta Cryst.. A61, C234-C235 (Abstracts of the XX IUCr Congress).

Francesca Malerba - Postdoctoral Fellow

Marcello Ceci - Postdoctoral Fellow

Annalisa Manca, Ph.D.

In the nervous system proteins are transported in axons from the cell body to the target areas. Failures in axonal transport have been related to neurodegenerative diseases as Alzheimer´s. Little is known about the anterograde transport mechanism of protein complexes along the axons and how this process is linked to neurodegenerative diseases. We focus our attention to the mechanisms underlying the axonal transport of proteins that ultimately are activated at the synapse and carry target-derived signals to the soma.

Quartu M., Serra M.P., Manca A., Mascia F., Follesa P., Del Fiacco M. (2005) Neurturin, persephin, and artemin in the human pre- and full-term newborn and adult hippocampus and fascia dentata. Brain Res. 1041, 157-66.

Quartu M., Serra M.P., Manca A., Follesa P., Ambu R., Del Fiacco M. (2003) Trk receptor-like immunoreactivity in the human pre-term newborn, infant and adult cerebellum. Int. J. Devl. Neurosci. 21, 309-320.

Mascia M.P., Biggio F., Mancuso L., Cabras S., Cocco P.L., Gorini G., Manca A., Purdy R., Follesa P., Biggio G. (2002) Changes in GABAA receptor gene expression induced by withdrawal of, but not long-term exposure to, ganaxolone in cultured rat cerebellar granule cells. J. Pharmacol. Exp. Ther. 303, 1014-1020.

Gianluca Amato - Scientist

Agnese Lecci - Ph.D. Student